Journal: ACS Nano

Article Title: Carrier-Free Nanoassembly Suppresses Phase Separation via Ribosome-Inspired Crowding Control for Enhanced Chemo-Immunotherapy

doi: 10.1021/acsnano.5c17081

Figure Lengend Snippet: Mechanism of tumor growth inhibition by OSI-027. (A) Apoptosis of PDAC cells by the OSI-027-treated THP-1-cell system and (B) quantification of the positive rate of apoptosis as a percentage. (C) Phagocytosis of PDAC cells by the OSI-027-treated THP-1-cell system and (D) quantification of the positive rate of phagocytosis as a percentage. (E) A schematic of the HUVEC culture supernatant of the OSI-027-treated coculture system. (F) Microvascular density of the PDAC cell and THP-1-cell coculture system after OSI-027 treatment and (G) quantification of the area ratio of the microvascular density percentages. Data are shown as mean ± SD ( n = 3). (* p < 0.05, ** p < 0.01 and *** p < 0.001).

Article Snippet: The mTOR inhibitor (OSI-027) and Annexin V/propidium iodide (PI) apoptosis assay kits were purchased from MedChemExpress (NJ, USA).

Techniques: Inhibition

Journal: Nature Communications

Article Title: Fusobacterium periodonticum promotes colorectal tumorigenesis via decanoic acid-driven neutrophil chemotaxis

doi: 10.1038/s41467-026-74591-y

Figure Lengend Snippet: A Flow cytometry analysis of neutrophil apoptosis after coculture with sodium decanoate (DeaNa), sodium palmitate (PaNa), and PBS. Quantitative graphs showing the proportion of late apoptotic/necrotic (Annexin V⁺/PI⁺) neutrophils ( B ) and early apoptotic (Annexin V⁺/PI⁻) neutrophils ( C ) under different treatments ( n = 3). D Schematic representation of neutrophil migration in vitro for 3 h at 37 °C. Created in BioRender. Yang, Q. (2026) https://BioRender.com/fm3t1j5 . Representative images of migrated neutrophils on the lower membrane stained with DAPI and photographed using the multifunctional enzyme reader. E Numbers of migrating neutrophils ( n = 3). F Volcano plot of differential gene expression in sodium decanoate-treated and PBS-treated neutrophils. G Heatmap showing the DEGs identified via RNA-seq in the presence of decanoic acid or PBS. H Quantitative reverse transcription PCR (qRT‒PCR) validation of gene expression changes in neutrophils treated with decanoic acid, palmitic acid and PBS ( n = 4). I Cell supernatant CXCL8 expression in neutrophils treated with decanoic acid, palmitic acid and PBS, as determined by ELISA ( n = 4). J Simplified schematic illustrating the coculture assay of neutrophils with Caco-2 cells, detailing the experimental setup. Created in BioRender. Yang, Q. (2026) https://BioRender.com/74ys0y4 . K qRT‒PCR validation of gene expression changes in Caco-2 cells after coculture with different fatty-acid-treated neutrophils ( n = 3). Data are shown as mean ± SEM, with representative results from at least three independent experiments. P values were determined by one-way ANOVA with Holm–Šidák test ( B , C ), one-way ANOVA with Dunnett’s test ( E , K ), one-way ANOVA with Tukey test ( H – I ). DeaNa, sodium decanoate; PaNa, sodium palmitate. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 and ns P > 0.05. Exact P values: B DeaNa vs PBS, P = 0.0465, PaNa vs PBS, P = 0.0002; C DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P < 0.0001; E DeaNa vs PBS, P = 0.0342, PaNa vs PBS, P = 0.4726; H Cxcl2 , DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0001; Cxcl8 , DeaNa vs PBS, P = 0.0120, PaNa vs PBS, P = 0.0725; Cpt1a , DeaNa vs PBS, P = 0.0441, PaNa vs PBS, P < 0.0001; Osm , DeaNa vs PBS, P = 0.0078, PaNa vs PBS, P = 0.0019; Vegfa , DeaNa vs PBS, P = 0.0319, PaNa vs PBS, P = 0.0009. I DeaNa vs PBS, P = 0.0016, PaNa vs PBS, P = 0.0101. K CXCL8, DeaNa vs PBS, P < 0.0001, PaNa vs PBS, P = 0.9138; OSM, DeaNa vs PBS, P = 0.0865, PaNa vs PBS, P = 0.0031; VEGFA, DeaNa vs PBS, P = 0.0053, PaNa vs PBS, P < 0.0001; AREG, DeaNa vs PBS, P = 0.0451, PaNa vs PBS, P = 0.0391; PTGS2, DeaNa vs PBS, P = 0.0015, PaNa vs PBS, P = 0.0224.

Article Snippet: After treatment, cells were collected and stained using an Annexin V-FITC/Propidium Iodide (PI)-PE Apoptosis Detection Kit (MedChemExpress, USA).

Techniques: Flow Cytometry, Migration, In Vitro, Membrane, Staining, Gene Expression, RNA Sequencing, Reverse Transcription, Biomarker Discovery, Expressing, Enzyme-linked Immunosorbent Assay, Co-culture Assay